{"id":431,"date":"2021-06-07T10:45:26","date_gmt":"2021-06-07T07:45:26","guid":{"rendered":"https:\/\/www.epigendx.online\/?p=431"},"modified":"2021-06-24T10:35:05","modified_gmt":"2021-06-24T07:35:05","slug":"fam19a4","status":"publish","type":"post","link":"https:\/\/www.epigendx.online\/en\/2021\/fam19a4\/","title":{"rendered":"Study of the methylation of RCGY sites in the regulatory region of the FAM19A4 gene in human cell line dna preparations by GLAD-PCR assay"},"content":{"rendered":"

[vc_row css=”.vc_custom_1576496196814{background-color: #ededed !important;}”][vc_column width=”2\/3″][vc_column_text]<\/p>\n

A.G. Akishev1<\/sup>, N.A.Netesova2<\/sup>, M.A. Abdurashitov1<\/sup>, S.Kh. Degtyarev1<\/sup><\/strong><\/p>\n

1<\/sup>\u00a0SibEnzyme, Novosibirsk
\n2<\/sup>\u00a0Epigenlab, Novosibirsk<\/p>\n

* corresponding author: Alexander Akishev, SibEnzyme, 2\/12 Ak.Timakov Str., Novosibirsk 630117, Russia; Tel: +7 383 3334991; Fax: +7 383 3336853; E-mail:\u00a0aki@sibenzyme.ru<\/a><\/p>\n

\n

The method of GLAD-PCR analysis was used to determine the methylation of RCGY sites in the regulatory region of the FAM19A4 gene (Xp3: 68932451 – 68932800) in the DNA preparations of the malignant human cell lines Raji, Jurkat, HeLa, U-937 and the control DNA of the lung fibroblast cell line L68.
\nIt is shown that the octanucleotide GCGCGCGC (Xp3: 68932500), located in the first exon of the FAM19A4 gene, is cleaved approximately equally in the DNA of all 4 malignant human cell lines, whereas in the DNA of L68 this site is hydrolyzed 10-15 times weaker. These results are consistent with data of epigenomic sequencing of L68, Raji, and U-937 DNA.
\nMethylation of CG-dinucleotides at positions 68932727 and\/or 68932740 in the promoter of the FAM19A4 gene occurs only in tumor DNA and is not observed in L68 DNA, whereas methylation of CG-dinucleotide at position 68932704 is observed only in HeLa DNA.<\/p>\n<\/div>\n

[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]Keywords:<\/strong> DNA methylation,\u00a0 human blood DNA, GLAD-PCR assay, the FAM19A4 gene<\/p>\n

DOI:<\/strong> 10.26213\/SE.2021.97.62.001<\/a><\/p>\n

Citation:<\/strong><\/p>\n

Akishev A.G., Netesova N.A., Abdurashitov M.A., Degtyarev S.Kh. (2021) Study of the methylation of RCGY sites in the regulatory region of the FAM19A4 gene in human cell line dna preparations by GLAD-PCR assay, Epigenet DNA diagnx, vol.2021(1), DOI: 10.26213\/SE.2021.97.62.001<\/a><\/p>\n

\"\u041b\u0438\u0446\u0435\u043d\u0437\u0438\u044f<\/a>
\nThis work is available at\u00a0Creative Commons licence \u00abAttribution-NonCommercial\u00bb\u00a0 4.0 International<\/a>.<\/p>\n

Full-text-pdf<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

This article is available only on Russian<\/h1>\n

[\/vc_column_text][vc_column_text]<\/p>\n

\u0420\u0438\u0441. 1. \u041d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043d\u0430\u044f \u043f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u044c \u0440\u0435\u0433\u0443\u043b\u044f\u0442\u043e\u0440\u043d\u043e\u0439 \u043e\u0431\u043b\u0430\u0441\u0442\u0438 \u0433\u0435\u043d\u0430 FAM19A4.<\/h4>\n

\u0420\u0430\u0439\u043e\u043d\u044b \u0441\u0432\u044f\u0437\u044b\u0432\u0430\u043d\u0438\u044f \u0433\u0435\u043d\u043e\u043c\u043d\u044b\u0445 \u043f\u0440\u0430\u0439\u043c\u0435\u0440\u043e\u0432 \u0432\u044b\u0434\u0435\u043b\u0435\u043d\u044b \u0436\u0435\u043b\u0442\u044b\u043c \u0438 \u0441\u0438\u043d\u0438\u043c \u0444\u043e\u043d\u043e\u043c, \u0430 \u0437\u043e\u043d\u0430 \u0441\u0432\u044f\u0437\u044b\u0432\u0430\u043d\u0438\u044f \u0444\u043b\u044e\u043e\u0440\u0435\u0441\u0446\u0435\u043d\u0442\u043d\u043e-\u043c\u0435\u0447\u0435\u043d\u044b\u0445 \u0437\u043e\u043d\u0434\u043e\u0432 \u0432\u044b\u0434\u0435\u043b\u0435\u043d\u0430 \u0437\u0435\u043b\u0435\u043d\u044b\u043c \u0446\u0432\u0435\u0442\u043e\u043c. \u0421\u0430\u0439\u0442\u044b RCGY, \u0443\u0437\u043d\u0430\u0432\u0430\u0435\u043c\u044b\u0435 \u0432 \u0441\u043b\u0443\u0447\u0430\u0435 \u0438\u0445 \u043c\u0435\u0442\u0438\u043b\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u0444\u0435\u0440\u043c\u0435\u043d\u0442\u043e\u043c GlaI, \u043f\u043e\u0434\u0447\u0435\u0440\u043a\u043d\u0443\u0442\u044b \u0434\u0432\u043e\u0439\u043d\u043e\u0439 \u043b\u0438\u043d\u0438\u0435\u0439. \u0421\u0430\u0439\u0442\u044b RCGY, \u0431\u043b\u0438\u0436\u0430\u0439\u0448\u0438\u0435 \u043a \u043f\u0430\u0440\u0430\u043c \u043f\u0440\u0430\u0439\u043c\u0435\u0440-\u0437\u043e\u043d\u0434, \u043e\u0431\u043e\u0437\u043d\u0430\u0447\u0435\u043d\u044b \u0448\u0440\u0438\u0444\u0442\u043e\u043c \u043a\u0440\u0430\u0441\u043d\u043e\u0433\u043e \u0446\u0432\u0435\u0442\u0430. CG-\u0434\u0438\u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u044b, \u043f\u0440\u043e\u0432\u0435\u0440\u044f\u0435\u043c\u044b\u0435 \u0441 \u043f\u043e\u043c\u043e\u0449\u044c\u044e \u0447\u0438\u043f\u0430 Illumina Infinium 450k \u0438 \u043d\u0430\u0445\u043e\u0434\u044f\u0449\u0438\u0435\u0441\u044f \u0432 \u0434\u0430\u043d\u043d\u043e\u043c \u0444\u0440\u0430\u0433\u043c\u0435\u043d\u0442\u0435 \u0414\u041d\u041a, \u0432\u044b\u0434\u0435\u043b\u0435\u043d\u044b \u0441\u0435\u0440\u044b\u043c \u0444\u043e\u043d\u043e\u043c (\u043f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e cg12417685, cg12219082, cg12412079, cg13921352). \u041b\u0438\u043b\u043e\u0432\u044b\u043c \u0446\u0432\u0435\u0442\u043e\u043c \u0432\u044b\u0434\u0435\u043b\u0435\u043d \u043d\u0430\u0447\u0430\u043b\u044c\u043d\u044b\u0439 \u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434 \u0433\u0435\u043d\u0430 \u043f\u043e \u0441\u0438\u0441\u0442\u0435\u043c\u0435 GenCode.[\/vc_column_text][vc_single_image image=”434″ img_size=”large” onclick=”link_image”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0420\u0438\u0441. 2.\u00a0 \u041a\u0430\u0440\u0442\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u0435 \u0440\u0438\u0434\u043e\u0432, \u043f\u043e\u043b\u0443\u0447\u0435\u043d\u043d\u044b\u0445\u00a0 \u043f\u0440\u0438 \u0441\u0435\u043a\u0432\u0435\u043d\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u0438 GlaI-\u0444\u0440\u0430\u0433\u043c\u0435\u043d\u0442\u043e\u0432 \u0433\u0435\u043d\u043e\u043c\u043d\u044b\u0445 \u0414\u041d\u041a L68, Raji \u0438 U-937\u00a0\u00a0 \u0432 \u043f\u0440\u043e\u043c\u043e\u0442\u043e\u0440\u043d\u043e\u0439 \u043e\u0431\u043b\u0430\u0441\u0442\u0438 \u0433\u0435\u043d\u0430 FAM19A4.<\/h4>\n

\u0416\u0435\u043b\u0442\u043e\u0439 \u0438 \u0433\u043e\u043b\u0443\u0431\u043e\u0439 \u0441\u0442\u0440\u0435\u043b\u043a\u0430\u043c\u0438 \u0438 \u0437\u0435\u043b\u0435\u043d\u044b\u043c\u0438 \u043f\u0440\u044f\u043c\u043e\u0443\u0433\u043e\u043b\u044c\u043d\u0438\u043a\u0430\u043c\u0438 \u043e\u0431\u043e\u0437\u043d\u0430\u0447\u0435\u043d\u044b \u0437\u043e\u043d\u044b \u0441\u0432\u044f\u0437\u044b\u0432\u0430\u043d\u0438\u044f \u0433\u0435\u043d\u043e\u043c\u043d\u044b\u0445 \u043f\u0440\u0430\u0439\u043c\u0435\u0440\u043e\u0432 \u0438 \u0444\u043b\u044e\u043e\u0440\u0435\u0441\u0446\u0435\u043d\u0442\u043d\u043e-\u043c\u0435\u0447\u0435\u043d\u044b\u0445 \u0437\u043e\u043d\u0434\u043e\u0432, \u0441\u043e\u043e\u0442\u0432\u0435\u0442\u0441\u0442\u0432\u0435\u043d\u043d\u043e.[\/vc_column_text][vc_single_image image=”435″ img_size=”large” alignment=”center” onclick=”img_link_large” img_link_target=”_blank”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0420\u0438\u0441. 3. \u041a\u0440\u0438\u0432\u044b\u0435 \u0444\u043b\u044e\u043e\u0440\u0435\u0441\u0446\u0435\u043d\u0446\u0438\u0438 \u0434\u043b\u044f GLAD-\u041f\u0426\u0420 \u0441 \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u043e\u0432\u0430\u043d\u0438\u0435\u043c \u0437\u043e\u043d\u0434\u0430 FAM19A4z641, \u0433\u0435\u043d\u043e\u043c\u043d\u043e\u0433\u043e \u043f\u0440\u0430\u0439\u043c\u0435\u0440\u0430 FAM19A4_618\u00a0\u0438 \u0433\u0438\u0431\u0440\u0438\u0434\u043d\u043e\u0433\u043e \u043f\u0440\u0430\u0439\u043c\u0435\u0440\u0430 HP10.<\/h4>\n

\u0421\u043f\u0440\u0430\u0432\u0430 \u0443\u043a\u0430\u0437\u0430\u043d\u044b \u0414\u041d\u041a-\u043c\u0430\u0442\u0440\u0438\u0446\u044b, \u0441\u043e\u043e\u0442\u0432\u0435\u0442\u0441\u0442\u0432\u0443\u044e\u0449\u0438\u0435 \u043a\u0440\u0438\u0432\u044b\u043c.[\/vc_column_text][vc_single_image image=”436″ img_size=”large” alignment=”center” onclick=”img_link_large” img_link_target=”_blank”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0422\u0430\u0431\u043b\u0438\u0446\u0430 1<\/strong><\/h4>\n

\u0417\u043d\u0430\u0447\u0435\u043d\u0438\u044f Cq \u0432 GLAD-\u041f\u0426\u0420 \u0430\u043d\u0430\u043b\u0438\u0437\u0435 \u0414\u041d\u041a \u043f\u044f\u0442\u0438 \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u044b\u0445 \u043b\u0438\u043d\u0438\u0439 \u0441 \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u043e\u0432\u0430\u043d\u0438\u0435\u043c \u0440\u0430\u0437\u043b\u0438\u0447\u043d\u044b\u0445 \u0441\u043e\u0447\u0435\u0442\u0430\u043d\u0438\u0439 \u0437\u043e\u043d\u0434\u0430 \u0438 \u0433\u0438\u0431\u0440\u0438\u0434\u043d\u044b\u0445 \u043f\u0440\u0430\u0439\u043c\u0435\u0440\u043e\u0432.[\/vc_column_text][vc_single_image image=”437″ img_size=”large” alignment=”center”][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"

The method of GLAD-PCR analysis was used to determine the methylation of RCGY sites in the regulatory region of the FAM19A4 gene (Xp3: 68932451 – 68932800) in the DNA preparations of the malignant human cell lines Raji, Jurkat, HeLa, U-937 and the control DNA of the lung fibroblast cell line L68.
\nIt is shown that the octanucleotide GCGCGCGC (Xp3: 68932500), located in the first exon of the FAM19A4 gene, is cleaved approximately equally in the DNA of all 4 malignant human cell lines, whereas in the DNA of L68 this site is hydrolyzed 10-15 times weaker. These results are consistent with data of epigenomic sequencing of L68, Raji, and U-937 DNA.
\nMethylation of CG-dinucleotides at positions 68932727 and\/or 68932740 in the promoter of the FAM19A4 gene occurs only in tumor DNA and is not observed in L68 DNA, whereas methylation of CG-dinucleotide at position 68932704 is observed only in HeLa DNA.<\/p>\n","protected":false},"author":1,"featured_media":440,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[33,4,28],"tags":[34,35,29],"coauthors":[16,15,17,26,12],"_links":{"self":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/431"}],"collection":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/comments?post=431"}],"version-history":[{"count":21,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/431\/revisions"}],"predecessor-version":[{"id":490,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/431\/revisions\/490"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media\/440"}],"wp:attachment":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media?parent=431"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/categories?post=431"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/tags?post=431"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/coauthors?post=431"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}