{"id":398,"date":"2021-05-06T09:53:48","date_gmt":"2021-05-06T06:53:48","guid":{"rendered":"https:\/\/www.epigendx.online\/?p=398"},"modified":"2021-06-08T12:30:11","modified_gmt":"2021-06-08T09:30:11","slug":"snp2","status":"publish","type":"post","link":"https:\/\/www.epigendx.online\/en\/2021\/snp2\/","title":{"rendered":"Determination of SNP 5mC\/T in position Chr1: 245618129  in DNA samples from the human blood by GlaI- and FatI-PCR assay"},"content":{"rendered":"<p>[vc_row css=&#8221;.vc_custom_1576496196814{background-color: #ededed !important;}&#8221;][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<p class=\"citation_authors\"><strong>A.G. Akishev<sup>1<\/sup>, N.A.Netesova<sup>2<\/sup>, M.A. Abdurashitov<sup>1<\/sup>, S.Kh. Degtyarev<sup>1<\/sup><\/strong><\/p>\n<p><sup>1<\/sup> SibEnzyme, Novosibirsk<br \/>\n<sup>2<\/sup> Epigenlab, Novosibirsk<\/p>\n<p>* corresponding author: Alexander Akishev, SibEnzyme, 2\/12 Ak.Timakov Str., Novosibirsk 630117, Russia; Tel: +7 383 3334991; Fax: +7 383 3336853; E-mail:\u00a0<a href=\"mailto:aki@sibenzyme.ru\">aki@sibenzyme.ru<\/a><\/p>\n<p>SNP 5mC\/T (Chr1: 245618129) in DNA samples from the human blood of 92donors was studied by GlaI and FatI-PCR assay. The work includes a) isolation of DNA from the blood cells, b) GlaI and FatI-PCR assay of DNA fragment Chr1: 245617889 &#8211; 245618464, c) determination of 5mC (Chr1: 245618129) and thymine in DNA preparations and d) comparative analysis of the obtained results.<\/p>\n<p>It was shown that in position (Chr1: 245618129) 43 donors (46,74%) have a heterozygote (5mC\/T), 28 donors (30,43%) have homozygote (T\/T) and 21 donors (22,83%) have homozygote (5mC\/5mC). Thus, taking into account a diploid set of chromosomes in the blood cells, SNP 5mC\/T is observed in 99 positions from 184 (53,8%).<\/p>\n<p>The results obtained have shown that cytosine in position Chr1: 245618129 in the most of the blood DNA is methylated.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_column_text]<strong>Keywords:<\/strong> SNP 5mC\/T analysis, DNA from human blood, GlaI-PCR assay, FatI-PCR assay<\/p>\n<p><strong>DOI:<\/strong> <a href=\"https:\/\/doi.org\/10.26213\/SE.2019.69.42836\" target=\"_blank\" rel=\"noopener noreferrer\">10.26213\/SE.2019.69.42836<\/a><\/p>\n<p><strong>Citation::<\/strong><\/p>\n<p>Akishev A.G., Netesova N.A., Abdurashitov M.A., Degtyarev S.Kh. Determination of SNP 5mC\/T in position Chr1: 245618129 in DNA samples from the human blood by GlaI- and FatI-PCR assay. Epigenet DNA diagnx, vol.1, 2021, p.13-23, DOI: <a href=\"https:\/\/doi.org\/10.26213\/SE.2019.69.42836\" target=\"_blank\" rel=\"noopener noreferrer\">10.26213\/SE.2019.69.42836<\/a><\/p>\n<p><a href=\"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/legalcode\" target=\"_blank\" rel=\"license noopener noreferrer\"><img style=\"border-width: 0;\" src=\"https:\/\/i.creativecommons.org\/l\/by-nc-nd\/4.0\/88x31.png\" alt=\"\u041b\u0438\u0446\u0435\u043d\u0437\u0438\u044f Creative Commons\" \/><\/a><br \/>\nThis work is available at\u00a0<a href=\"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/legalcode\" rel=\"license\">Creative Commons licence \u00abAttribution-NonCommercial\u00bb\u00a0 4.0 International<\/a>.<\/p>\n<p><a href=\"https:\/\/www.epigendx.online\/wp-content\/uploads\/2021\/05\/snp2_rus.pdf\" target=\"_blank\" rel=\"noopener\">Full-text-pdf<\/a><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h3><strong>Sorry. Full text is available only on Russian<\/strong><\/h3>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"<p>SNP 5mC\/T (Chr1: 245618129) in DNA samples from the human blood of 92donors was studied by GlaI and FatI-PCR assay. The work includes a) isolation of DNA from the blood cells, b) GlaI and FatI-PCR assay of DNA fragment Chr1: 245617889 &#8211; 245618464, c) determination of 5mC (Chr1: 245618129) and thymine in DNA preparations and d) comparative analysis of the obtained results.<\/p>\n<p>It was shown that in position (Chr1: 245618129) 43 donors (46,74%) have a heterozygote (5mC\/T), 28 donors (30,43%) have homozygote (T\/T) and 21 donors (22,83%) have homozygote (5mC\/5mC). Thus, taking into account a diploid set of chromosomes in the blood cells, SNP 5mC\/T is observed in 99 positions from 184 (53,8%).<\/p>\n<p>The results obtained have shown that cytosine in position Chr1: 245618129 in the most of the blood DNA is methylated.<\/p>\n","protected":false},"author":1,"featured_media":418,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[5,33,4,28],"tags":[37,29,30],"coauthors":[12],"_links":{"self":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/398"}],"collection":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/comments?post=398"}],"version-history":[{"count":12,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/398\/revisions"}],"predecessor-version":[{"id":423,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/398\/revisions\/423"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media\/418"}],"wp:attachment":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media?parent=398"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/categories?post=398"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/tags?post=398"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/coauthors?post=398"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}