{"id":254,"date":"2021-04-13T07:16:50","date_gmt":"2021-04-13T04:16:50","guid":{"rendered":"https:\/\/www.epigendx.online\/?p=254"},"modified":"2021-05-10T09:13:04","modified_gmt":"2021-05-10T06:13:04","slug":"snp","status":"publish","type":"post","link":"https:\/\/www.epigendx.online\/en\/2021\/snp\/","title":{"rendered":"Determination of SNP 5mC\/T in AluSx repeat (Chr16: 75033884) in DNA samples from the human blood by GlaI- and FatI-PCR assay"},"content":{"rendered":"

[vc_row css=”.vc_custom_1576496196814{background-color: #ededed !important;}”][vc_column width=”2\/3″][vc_column_text]<\/p>\n

A.G. Akishev1<\/sup>, N.A.Netesova2<\/sup>, M.A. Abdurashitov1<\/sup>, S.Kh. Degtyarev1<\/sup><\/strong><\/p>\n

1<\/sup> SibEnzyme, Novosibirsk
\n2<\/sup> Epigenlab, Novosibirsk<\/p>\n

* corresponding author: Alexander Akishev, SibEnzyme, 2\/12 Ak.Timakov Str., Novosibirsk 630117, Russia; Tel: +7 383 3334991; Fax: +7 383 3336853; E-mail:\u00a0aki@sibenzyme.ru<\/a><\/p>\n

SNP 5mC\/T in AluSx repeat (Chr16: 75033884) in DNA samples from the human blood of 92donors was studied by GlaI and FatI-PCR assay. The work includes a) isolation of DNA from the blood cells, b) GlaI and FatI-PCR assay of DNA fragment Chr16: 75033860 \u2013 75033957, c) determination of 5mC (Chr16: 75033884) and thymine (Chr16: 75033884) \u0432 in DNA preparations and d) comparative analysis of the obtained results.<\/p>\n

It was shown that in position (Chr16: 75033884) 53 donors (57,61%) have a heterozygote (5mC-T), 19 donors (20,65%) have homozygote (T-T) and 20 donors (21,74%) have homozygote (5mC-5mC). Thus, taking into account a diploid set of chromosomes in the blood cells, SNP 5mC\/T is observed in 70 positions from 156 (49,46%).<\/p>\n

The results obtained have shown that cytosine in position Chr16: 75033884 in the most of the blood DNA is methylated in accordance with a literature data about a significant methylation of CG-dinucleotides in Alu-repeats.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]Keywords:<\/strong> SNP 5mC\/T analysis, DNA from human blood, GlaI-PCR assay, FatI-PCR assay<\/p>\n

DOI:<\/strong> 10.26213\/SE.2021.11.76.001<\/a><\/p>\n

Citation::<\/strong><\/p>\n

A.G. Akishev, N.A.Netesova, M.A. Abdurashitov, S.Kh. Degtyarev Determination of SNP 5mC\/T in AluSx repeat (Chr16: 75033884) in DNA samples from the human blood by GlaI- and FatI-PCR assay. Epigenet DNA diagnx, vol.1, 2021, DOI: 10.26213\/SE.2021.11.76.001<\/a><\/p>\n

\"\u041b\u0438\u0446\u0435\u043d\u0437\u0438\u044f<\/a>
\nThis work is available at\u00a0Creative Commons licence \u00abAttribution-NonCommercial\u00bb\u00a0 4.0 International<\/a>.<\/p>\n

Full-text-pdf<\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Sorry. Full text is available only on Russian<\/strong><\/h3>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0420\u0438\u0441\u0443\u043d\u043e\u043a 1.<\/h4>\n

\u041d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043d\u0430\u044f \u043f\u043e\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u044c \u0430\u043d\u0430\u043b\u0438\u0437\u0438\u0440\u0443\u0435\u043c\u043e\u0433\u043e \u0443\u0447\u0430\u0441\u0442\u043a\u0430 \u0433\u0435\u043d\u043e\u043c\u043d\u043e\u0439 \u0414\u041d\u041a<\/p>\n

[\/vc_column_text][vc_single_image image=”256″ img_size=”large”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0422\u0430\u0431\u043b\u0438\u0446\u0430 1.<\/h4>\n

\u041f\u043e\u043b \u0438 \u0432\u043e\u0437\u0440\u0430\u0441\u0442 \u0434\u043e\u043d\u043e\u0440\u043e\u0432 \u043a\u0440\u043e\u0432\u0438, \u043a\u043e\u043d\u0446\u0435\u043d\u0442\u0440\u0430\u0446\u0438\u044f \u0438 \u0444\u043e\u0442\u043e\u043c\u0435\u0442\u0440\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0447\u0438\u0441\u0442\u043e\u0442\u0430 \u043f\u043e\u043b\u0443\u0447\u0435\u043d\u043d\u043e\u0439 \u043b\u0435\u0439\u043a\u043e\u0446\u0438\u0442\u0430\u0440\u043d\u043e\u0439 \u0414\u041d\u041a<\/p>\n

 <\/p>\n

[\/vc_column_text][vc_column_text]\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
\u00a0N \u0434\u043e\u043d\u043e\u0440\u0430 <\/th>\u041f\u043e\u043b<\/th>\u0412\u043e\u0437\u0440\u0430\u0441\u0442, \u043b\u0435\u0442<\/th>\u041a\u043e\u043d\u0446\u0435\u043d\u0442\u0440\u0430\u0446\u0438\u044f \u0414\u041d\u041a, \u043d\u0433\/\u043c\u043a\u043b<\/th>\u0410260\/\u0410280<\/th> <\/th>\u00a0N \u0434\u043e\u043d\u043e\u0440\u0430 <\/th>\u041f\u043e\u043b<\/th>\u0412\u043e\u0437\u0440\u0430\u0441\u0442, \u043b\u0435\u0442<\/th>\u041a\u043e\u043d\u0446\u0435\u043d\u0442\u0440\u0430\u0446\u0438\u044f \u0414\u041d\u041a, \u043d\u0433\/\u043c\u043a\u043b<\/th>\u0410260\/\u0410280<\/th>\n<\/tr>\n<\/thead>\n
1172<\/td>\u0436\u0435\u043d<\/td>14<\/td>376<\/td>1.60<\/td><\/td>1111<\/td>\u0436\u0435\u043d<\/td>50<\/td>141<\/td>1.70<\/td>\n<\/tr>\n
1164<\/td>\u0436\u0435\u043d<\/td>17<\/td>252<\/td>1.70<\/td><\/td>1151<\/td>\u0436\u0435\u043d<\/td>50<\/td>225<\/td>1.70<\/td>\n<\/tr>\n
1147<\/td>\u043c\u0443\u0436<\/td>18<\/td>180<\/td>2.00<\/td><\/td>1087<\/td>\u0436\u0435\u043d<\/td>51<\/td>95<\/td>1.70<\/td>\n<\/tr>\n
1133<\/td>\u043c\u0443\u0436<\/td>18<\/td>55<\/td>1.70<\/td><\/td>1093<\/td>\u043c\u0443\u0436<\/td>51<\/td>201<\/td>1.70<\/td>\n<\/tr>\n
1187<\/td>\u043c\u0443\u0436<\/td>20<\/td>304<\/td>1.80<\/td><\/td>1129<\/td>\u0436\u0435\u043d<\/td>51<\/td>72<\/td>1.70<\/td>\n<\/tr>\n
1502<\/td>\u0436\u0435\u043d<\/td>20<\/td>461<\/td>1.70<\/td><\/td>1165<\/td>\u043c\u0443\u0436<\/td>51<\/td>356<\/td>1.60<\/td>\n<\/tr>\n
1487<\/td>\u0436\u0435\u043d<\/td>22<\/td>406<\/td>1.70<\/td><\/td>584<\/td>\u043c\u0443\u0436<\/td>53<\/td>72<\/td>1.90<\/td>\n<\/tr>\n
1304<\/td>\u0436\u0435\u043d<\/td>23<\/td>129<\/td>1.70<\/td><\/td>1159<\/td>\u043c\u0443\u0436<\/td>53<\/td>358<\/td>1.70<\/td>\n<\/tr>\n
1218<\/td>\u043c\u0443\u0436<\/td>24<\/td>317<\/td>1.70<\/td><\/td>1358<\/td>\u043c\u0443\u0436<\/td>53<\/td>784<\/td>1.80<\/td>\n<\/tr>\n
1379<\/td>\u0436\u0435\u043d<\/td>24<\/td>241<\/td>1.80<\/td><\/td>587<\/td>\u0436\u0435\u043d<\/td>54<\/td>41<\/td>1.90<\/td>\n<\/tr>\n
1243<\/td>\u043c\u0443\u0436<\/td>24<\/td>280<\/td>1.70<\/td><\/td>561<\/td>\u043c\u0443\u0436<\/td>56<\/td>50<\/td>1.80<\/td>\n<\/tr>\n
1332<\/td>\u043c\u0443\u0436<\/td>25<\/td>507<\/td>1.90<\/td><\/td>1280<\/td>\u043c\u0443\u0436<\/td>56<\/td>436<\/td>1.80<\/td>\n<\/tr>\n
1173<\/td>\u0436\u0435\u043d<\/td>27<\/td>232<\/td>1.70<\/td><\/td>1127<\/td>\u0436\u0435\u043d<\/td>57<\/td>123<\/td>1.70<\/td>\n<\/tr>\n
1200<\/td>\u043c\u0443\u0436<\/td>27<\/td>271<\/td>1.60<\/td><\/td>1135<\/td>\u0436\u0435\u043d<\/td>57<\/td>127<\/td>1.60<\/td>\n<\/tr>\n
1203<\/td>\u043c\u0443\u0436<\/td>27<\/td>309<\/td>1.60<\/td><\/td>1163<\/td>\u0436\u0435\u043d<\/td>57<\/td>157<\/td>1.70<\/td>\n<\/tr>\n
1209<\/td>\u043c\u0443\u0436<\/td>27<\/td>204<\/td>1.70<\/td><\/td>1130<\/td>\u0436\u0435\u043d<\/td>58<\/td>59<\/td>1.70<\/td>\n<\/tr>\n
1214<\/td>\u0436\u0435\u043d<\/td>27<\/td>265<\/td>1.60<\/td><\/td>1137<\/td>\u0436\u0435\u043d<\/td>59<\/td>299<\/td>1.60<\/td>\n<\/tr>\n
1329<\/td>\u043c\u0443\u0436<\/td>27<\/td>488<\/td>1.80<\/td><\/td>1348<\/td>\u043c\u0443\u0436<\/td>60<\/td>761<\/td>1.70<\/td>\n<\/tr>\n
1225<\/td>\u043c\u0443\u0436<\/td>28<\/td>114<\/td>1.90<\/td><\/td>1349<\/td>\u043c\u0443\u0436<\/td>62<\/td>355<\/td>1.80<\/td>\n<\/tr>\n
1226<\/td>\u0436\u0435\u043d<\/td>30<\/td>297<\/td>1.90<\/td><\/td>1294<\/td>\u043c\u0443\u0436<\/td>63<\/td>173<\/td>1.60<\/td>\n<\/tr>\n
1221<\/td>\u0436\u0435\u043d<\/td>31<\/td>263<\/td>1.70<\/td><\/td>1369<\/td>\u0436\u0435\u043d<\/td>63<\/td>301<\/td>1.90<\/td>\n<\/tr>\n
1134<\/td>\u043c\u0443\u0436<\/td>32<\/td>53<\/td>1.70<\/td><\/td>1277<\/td>\u0436\u0435\u043d<\/td>64<\/td>330<\/td>1.70<\/td>\n<\/tr>\n
571<\/td>\u043c\u0443\u0436<\/td>33<\/td>30<\/td>1.80<\/td><\/td>1152<\/td>\u0436\u0435\u043d<\/td>65<\/td>112<\/td>1.70<\/td>\n<\/tr>\n
1161<\/td>\u043c\u0443\u0436<\/td>33<\/td>197<\/td>1.70<\/td><\/td>1205<\/td>\u043c\u0443\u0436<\/td>65<\/td>420<\/td>1.70<\/td>\n<\/tr>\n
1192<\/td>\u0436\u0435\u043d<\/td>33<\/td>110<\/td>1.70<\/td><\/td>237<\/td>\u043c\u0443\u0436<\/td>66<\/td>31<\/td>1.80<\/td>\n<\/tr>\n
1184<\/td>\u0436\u0435\u043d<\/td>34<\/td>305<\/td>1.70<\/td><\/td>d559<\/td>\u0436\u0435\u043d<\/td>66<\/td>37<\/td>1.70<\/td>\n<\/tr>\n
1208<\/td>\u043c\u0443\u0436<\/td>35<\/td>207<\/td>1.60<\/td><\/td>1143<\/td>\u0436\u0435\u043d<\/td>67<\/td>84<\/td>1.60<\/td>\n<\/tr>\n
604<\/td>\u0436\u0435\u043d<\/td>36<\/td>52<\/td>1.90<\/td><\/td>1428<\/td>\u043c\u0443\u0436<\/td>67<\/td>433<\/td>1.80<\/td>\n<\/tr>\n
1086<\/td>\u043c\u0443\u0436<\/td>36<\/td>193<\/td>1.70<\/td><\/td>1365<\/td>\u0436\u0435\u043d<\/td>68<\/td>204<\/td>1.80<\/td>\n<\/tr>\n
1217<\/td>\u043c\u0443\u0436<\/td>36<\/td>407<\/td>1.60<\/td><\/td>173<\/td>\u043c\u0443\u0436<\/td>68<\/td>352<\/td>1.70<\/td>\n<\/tr>\n
1247<\/td>\u0436\u0435\u043d<\/td>36<\/td>286<\/td>1.70<\/td><\/td>1437<\/td>\u043c\u0443\u0436<\/td>68<\/td>213<\/td>1.80<\/td>\n<\/tr>\n
1235<\/td>\u0436\u0435\u043d<\/td>37<\/td>168<\/td>1.70<\/td><\/td>240<\/td>\u043c\u0443\u0436<\/td>69<\/td>18<\/td>1.90<\/td>\n<\/tr>\n
1345<\/td>\u043c\u0443\u0436<\/td>37<\/td>420<\/td>1.80<\/td><\/td>1146<\/td>\u0436\u0435\u043d<\/td>70<\/td>149<\/td>1.80<\/td>\n<\/tr>\n
1094<\/td>\u0436\u0435\u043d<\/td>38<\/td>172<\/td>1.70<\/td><\/td>1413<\/td>\u043c\u0443\u0436<\/td>70<\/td>522<\/td>1.90<\/td>\n<\/tr>\n
1219<\/td>\u0436\u0435\u043d<\/td>41<\/td>315<\/td>1.80<\/td><\/td>40<\/td>\u0436\u0435\u043d<\/td>72<\/td>179<\/td>1.80<\/td>\n<\/tr>\n
1381<\/td>\u0436\u0435\u043d<\/td>41<\/td>500<\/td>1.70<\/td><\/td>205<\/td>\u0436\u0435\u043d<\/td>72<\/td>461<\/td>1.80<\/td>\n<\/tr>\n
1207<\/td>\u043c\u0443\u0436<\/td>42<\/td>220<\/td>1.60<\/td><\/td>1115<\/td>\u043c\u0443\u0436<\/td>72<\/td>78<\/td>1.70<\/td>\n<\/tr>\n
1323<\/td>\u043c\u0443\u0436<\/td>42<\/td>300<\/td>1.80<\/td><\/td>1576<\/td>\u043c\u0443\u0436<\/td>72<\/td>307<\/td>1.80<\/td>\n<\/tr>\n
1411<\/td>\u043c\u0443\u0436<\/td>42<\/td>569<\/td>1.80<\/td><\/td>178<\/td>\u043c\u0443\u0436<\/td>73<\/td>182<\/td>1.70<\/td>\n<\/tr>\n
1145<\/td>\u0436\u0435\u043d<\/td>45<\/td>323<\/td>1.60<\/td><\/td>1669<\/td>\u043c\u0443\u0436<\/td>74<\/td>351<\/td>1.70<\/td>\n<\/tr>\n
1171<\/td>\u0436\u0435\u043d<\/td>45<\/td>67<\/td>1.60<\/td><\/td>121<\/td>\u0436\u0435\u043d<\/td>75<\/td>163<\/td>1.70<\/td>\n<\/tr>\n
1491<\/td>\u0436\u0435\u043d<\/td>46<\/td>498<\/td>1.70<\/td><\/td>1168<\/td>\u0436\u0435\u043d<\/td>79<\/td>239<\/td>1.70<\/td>\n<\/tr>\n
603<\/td>\u0436\u0435\u043d<\/td>47<\/td>108<\/td>1.90<\/td><\/td>1174<\/td>\u0436\u0435\u043d<\/td>79<\/td>271<\/td>1.60<\/td>\n<\/tr>\n
1155<\/td>\u043c\u0443\u0436<\/td>48<\/td>110<\/td>1.60<\/td><\/td>1160<\/td>\u0436\u0435\u043d<\/td>80<\/td>195<\/td>1.70<\/td>\n<\/tr>\n
1338<\/td>\u043c\u0443\u0436<\/td>49<\/td>696<\/td>1.80<\/td><\/td>55<\/td>\u0436\u0435\u043d<\/td>81<\/td>221<\/td>1.70<\/td>\n<\/tr>\n
1099<\/td>\u043c\u0443\u0436<\/td>50<\/td>144<\/td>1.70<\/td><\/td>56<\/td>\u043c\u0443\u0436<\/td>81<\/td>414<\/td>1.70<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0420\u0438\u0441\u0443\u043d\u043e\u043a 2.<\/h4>\n

\u041a\u0440\u0438\u0432\u044b\u0435 \u0444\u043b\u044e\u043e\u0440\u0435\u0441\u0446\u0435\u043d\u0446\u0438\u0438 \u0434\u043b\u044f \u041f\u0426\u0420 \u0441 \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u043e\u0432\u0430\u043d\u0438\u0435\u043c \u0443\u0431\u044b\u0432\u0430\u044e\u0449\u0438\u0445 \u043a\u043e\u043b\u0438\u0447\u0435\u0441\u0442\u0432 HinfI-\u0444\u0440\u0430\u0433\u043c\u0435\u043d\u0442\u043e\u0432 \u0433\u0435\u043d\u043e\u043c\u043d\u043e\u0439 \u0414\u041d\u041a \u043e\u0431\u0440\u0430\u0437\u0446\u0430 1087 (\u0432\u0435\u0440\u0445\u043d\u0438\u0439 \u0440\u044f\u0434) \u0438 \u0433\u0435\u043d\u043e\u043c\u043d\u043e\u0439 \u0414\u041d\u041a \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u043e\u0439 \u043b\u0438\u043d\u0438\u0438 Raji (\u043d\u0438\u0436\u043d\u0438\u0439 \u0440\u044f\u0434) \u0438 \u0441\u0442\u0430\u043d\u0434\u0430\u0440\u0442\u043d\u044b\u0435 \u043a\u0440\u0438\u0432\u044b\u0435 \u0434\u043b\u044f \u043f\u0440\u043e\u0432\u0435\u0434\u0435\u043d\u043d\u044b\u0445 \u0440\u0435\u0430\u043a\u0446\u0438\u0439.<\/p>\n

[\/vc_column_text][vc_single_image image=”263″ img_size=”large” alignment=”center” onclick=”link_image”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0422\u0430\u0431\u043b\u0438\u0446\u0430 2.<\/h4>\n

\u0417\u043d\u0430\u0447\u0435\u043d\u0438\u044f Cq \u0432 \u0441\u043b\u0443\u0447\u0430\u0435 \u0433\u0438\u0434\u0440\u043e\u043b\u0438\u0437\u0430 \u0414\u041d\u041a \u043f\u043e \u0441\u0430\u0439\u0442\u0430\u043c GANTC (HinfI), CATG (Fat-\u041f\u0426\u0420 \u0430\u043d\u0430\u043b\u0438\u0437) \u0438 A(5mC)GC (GlaI-\u041f\u0426\u0420 \u0430\u043d\u0430\u043b\u0438\u0437) \u0438 \u0437\u043d\u0430\u0447\u0435\u043d\u0438\u044f \u0440\u0430\u0437\u043d\u0438\u0446\u044b \u0432\u0435\u043b\u0438\u0447\u0438\u043d\u044b Cq \u0434\u043b\u044f \u0434\u0432\u0443\u0445 \u043f\u043e\u0441\u043b\u0435\u0434\u043d\u0438\u0445 \u0440\u0435\u0430\u043a\u0446\u0438\u0439 \u043e\u0442\u043d\u043e\u0441\u0438\u0442\u0435\u043b\u044c\u043d\u043e \u043f\u0435\u0440\u0432\u043e\u0439 (\u0394Cq_T \u0438 \u0394Cq_5mC), \u0441\u043e\u043e\u0442\u0432\u0435\u0442\u0441\u0442\u0432\u0435\u043d\u043d\u043e.[\/vc_column_text][vc_single_image image=”285″ img_size=”large” alignment=”center”][vc_single_image image=”284″ img_size=”large” alignment=”center”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0420\u0438\u0441\u0443\u043d\u043e\u043a 3<\/h4>\n

\u0421\u0432\u0435\u0440\u0445\u0443<\/strong> \u2013 \u0417\u043d\u0430\u0447\u0435\u043d\u0438\u044f \u0394Cq_5mC \u0438 \u0394Cq_T Cq \u0434\u043b\u044f 92-\u0445 \u043e\u0431\u0440\u0430\u0437\u0446\u043e\u0432 \u0434\u043e\u043d\u043e\u0440\u0441\u043a\u043e\u0439 \u0414\u041d\u041a \u043b\u0435\u0439\u043a\u043e\u0446\u0438\u0442\u043e\u0432 (\u043a\u0440\u0430\u0441\u043d\u044b\u043c \u0438 \u0441\u0438\u043d\u0438\u043c \u0446\u0432\u0435\u0442\u043e\u043c, \u0441\u043e\u043e\u0442\u0432\u0435\u0442\u0441\u0442\u0432\u0435\u043d\u043d\u043e). \u0421\u043d\u0438\u0437\u0443<\/strong> – \u043f\u0440\u043e\u0446\u0435\u043d\u0442 \u043c\u043e\u043b\u0435\u043a\u0443\u043b \u0414\u041d\u041a, \u043d\u0435\u0440\u0430\u0441\u0449\u0435\u043f\u043b\u0435\u043d\u043d\u044b\u0445 \u0432 \u0445\u043e\u0434\u0435 GlaI- \u0438 FatI-\u041f\u0426\u0420 \u0430\u043d\u0430\u043b\u0438\u0437\u0430, \u043e\u0442\u043d\u043e\u0441\u0438\u0442\u0435\u043b\u044c\u043d\u043e \u043e\u0431\u0449\u0435\u0433\u043e \u043a\u043e\u043b\u0438\u0447\u0435\u0441\u0442\u0432\u043e \u043c\u043e\u043b\u0435\u043a\u0443\u043b \u0414\u041d\u041a, \u043e\u043f\u0440\u0435\u0434\u0435\u043b\u0435\u043d\u043d\u044b\u0445\u00a0 \u041f\u0426\u0420 \u0430\u043d\u0430\u043b\u0438\u0437\u043e\u043c HinfI-\u0433\u0438\u0434\u0440\u043e\u043b\u0438\u0437\u0430\u0442\u043e\u0432 (\u043a\u0440\u0430\u0441\u043d\u044b\u043c \u0438 \u0441\u0438\u043d\u0438\u043c \u0446\u0432\u0435\u0442\u043e\u043c, \u0441\u043e\u043e\u0442\u0432\u0435\u0442\u0441\u0442\u0432\u0435\u043d\u043d\u043e).<\/p>\n

[\/vc_column_text][vc_single_image image=”274″ img_size=”large” alignment=”center”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0422\u0430\u0431\u043b\u0438\u0446\u0430 3<\/h4>\n

\u0420\u0430\u0441\u043f\u0440\u0435\u0434\u0435\u043b\u0435\u043d\u0438\u0435 \u0434\u0438\u043f\u043b\u043e\u0438\u0434\u043d\u044b\u0445 \u043d\u0430\u0431\u043e\u0440\u043e\u0432 \u0430\u043b\u0435\u043b\u043b\u0435\u0439 \u0432 \u043e\u0431\u0449\u0435\u0439 \u0432\u044b\u0431\u043e\u0440\u043a\u0435 \u0438 \u043e\u0442\u0434\u0435\u043b\u044c\u043d\u043e \u043f\u043e \u043f\u043e\u043b\u0430\u043c.<\/p>\n

 <\/p>\n

[\/vc_column_text][vc_single_image image=”281″ img_size=”large” alignment=”center”][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1580187576648{background-color: #f4f4f4 !important;}”][vc_column][vc_column_text]<\/p>\n

\u0422\u0430\u0431\u043b\u0438\u0446\u0430 4<\/h4>\n

\u0417\u043d\u0430\u0447\u0435\u043d\u0438\u044f \u0394Cq \u0434\u043b\u044f \u0440\u044f\u0434\u0430 \u0433\u0435\u043d\u043e\u043c\u043d\u044b\u0445 \u0414\u041d\u041a \u043c\u0430\u043b\u0438\u0433\u043d\u0430\u043d\u0442\u043d\u044b\u0445 \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u044b\u0445 \u043b\u0438\u043d\u0438\u0439 \u0447\u0435\u043b\u043e\u0432\u0435\u043a\u0430 \u0438 \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u043e\u0439 \u043b\u0438\u043d\u0438\u0438 \u0444\u0438\u0431\u0440\u043e\u0431\u043b\u0430\u0441\u0442\u043e\u0432 \u043b\u0435\u0433\u043a\u0438\u0445 L68<\/p>\n

 <\/p>\n

 <\/p>\n

[\/vc_column_text][vc_single_image image=”282″ img_size=”large” alignment=”center”][\/vc_column][\/vc_row]<\/p>","protected":false},"excerpt":{"rendered":"

SNP 5mC\/T in AluSx repeat (Chr16: 75033884) in DNA samples from the human blood of 92donors was studied by GlaI and FatI-PCR assay. The work includes a) isolation of DNA from the blood cells, b) GlaI and FatI-PCR assay of DNA fragment Chr16: 75033860 \u2013 75033957, c) determination of 5mC (Chr16: 75033884) and thymine (Chr16: 75033884) \u0432 in DNA preparations and d) comparative analysis of the obtained results.
\nIt was shown that in position (Chr16: 75033884) 53 donors (57,61%) have a heterozygote (5mC-T), 19 donors (20,65%) have homozygote (T-T) and 20 donors (21,74%) have homozygote (5mC-5mC). Thus, taking into account a diploid set of chromosomes in the blood cells, SNP 5mC\/T is observed in 70 positions from 156 (49,46%).
\nThe results obtained have shown that cytosine in position Chr16: 75033884 in the most of the blood DNA is methylated in accordance with a literature data about a significant methylation of CG-dinucleotides in Alu-repeats<\/p>\n","protected":false},"author":1,"featured_media":354,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[5,33,4,28],"tags":[31,29,30],"coauthors":[16,17,15,26],"_links":{"self":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/254"}],"collection":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/comments?post=254"}],"version-history":[{"count":55,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/254\/revisions"}],"predecessor-version":[{"id":424,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/posts\/254\/revisions\/424"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media\/354"}],"wp:attachment":[{"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/media?parent=254"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/categories?post=254"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/tags?post=254"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/www.epigendx.online\/en\/wp-json\/wp\/v2\/coauthors?post=254"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}